Thirdhand smoke causes DNA damage

Thirdhand smoke causes DNA damage

June 20, 2013 — A study led by researchers from Lawrence Berkeley National Laboratory has found for the first time that thirdhand smoke -- the noxious residue that clings to virtually all surfaces long after the secondhand smoke from a cigarette has cleared out -- causes significant genetic damage in human cells.

Furthermore, the study also found that chronic exposure is worse than acute exposure, with the chemical compounds in samples exposed to chronic thirdhand smoke existing in higher concentrations and causing more DNA damage than samples exposed to acute thirdhand smoke, suggesting that the residue becomes more harmful over time.

"This is the very first study to find that thirdhand smoke is mutagenic," said Lara Gundel, a Berkeley Lab scientist and co-author of the study. "Tobacco-specific nitrosamines, some of the chemical compounds in thirdhand smoke, are among the most potent carcinogens there are. They stay on surfaces, and when those surfaces are clothing or carpets, the danger to children is especially serious."

Their paper, "Thirdhand smoke causes DNA damage in human cells," was published in the journal Mutagenesis. The lead investigator was Bo Hang, a biochemist in the Life Sciences Division of Berkeley Lab; he worked with an interdisciplinary group, including chemists from Berkeley Lab's Environmental Energy Technologies Division -- Gundel, Hugo Destaillats and Mohamad Sleiman -- as well as scientists from UC San Francisco, UCLA Medical Center and the University of Texas.

Berkeley Lab scientists (from left) Altaf Sarker, Mohamad Sleiman, Lara Gundel, Bo Hang and Hugo Destaillats worked on the thirdhand smoke study. (Photo by Roy Kaltschmidt/Berkeley Lab)

The researchers used two common in vitro assays, the Comet assay and the long amplicon-qPCR assay, to test for genotoxicity and found that thirdhand smoke can cause both DNA strand breaks and oxidative DNA damage, which can lead to gene mutation. Genotoxicity is associated with the development of diseases and is a critical mechanism responsible for many types of cancer caused by smoking and secondhand smoke exposure.

"Until this study, the toxicity of thirdhand smoke has not been well understood," Hang said. "Thirdhand smoke has a smaller quantity of chemicals than secondhand smoke, so it's good to have experimental evidence to confirm its genotoxicity."

It is the first major study of disease-related mechanisms to come out of the California Consortium on the Health Effects of Thirdhand Smoke, which was established two years ago largely as a result of work published in 2010 by Gundel, Destaillats, Sleiman and others. The Consortium is funded by the Tobacco-Related Disease Research Program, which is managed by the University of California and funded by state cigarette taxes.

The 2010 studies from Berkeley Lab found that residual nicotine can react with ozone and nitrous acid -- both common indoor air pollutants -- to form hazardous agents. When nicotine in thirdhand smoke reacts with nitrous acid it undergoes a chemical transformation and forms carcinogenic tobacco-specific nitrosamines, such as NNA, NNK and NNN. Nicotine can react with ozone to form ultrafine particles, which can carry harmful chemicals and pass through human tissue. Humans can be exposed to thirdhand smoke through inhalation, ingestion or skin contact.

Thirdhand smoke is particularly insidious because it is extremely difficult to eradicate. Studies have found that it can still be detected in dust and surfaces of apartments more than two months after smokers moved out. Common cleaning methods such as vacuuming, wiping and ventilation have not proven effective in lowering nicotine contamination. "You can do some things to reduce the odors, but it's very difficult to really clean it completely," said Destaillats. "The best solution is to substitute materials, such as change the carpet, repaint."

Now the new study suggests thirdhand smoke could become more harmful over time. To generate the samples, the researchers put paper strips in smoking chambers. The acute samples, generated at Berkeley Lab, were exposed to five cigarettes smoked in about 20 minutes, and the chronic samples, generated at UCSF, were exposed to cigarette smoke for 258 hours over 196 days. During that time, the chamber was also ventilated for about 35 hours.

The researchers found that the concentrations of more than half of the compounds studied were higher in the chronic samples than in the acute. They also found higher levels of DNA damage caused by the chronic samples. "The cumulative effect of thirdhand smoke is quite significant," Gundel said. "The findings suggest the materials could be getting more toxic with time."

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours. The concentrations of the compounds were carefully measured. "They are close to real-life concentrations, and in fact are on the lower side of what someone might be exposed to," Hang said.

Next Hang is pursuing further understanding of the chemistry of the NNA reaction with DNA bases. NNA is a tobacco-specific nitrosamine that is not found in freshly emitted secondhand smoke. "It looks like it's a very important component of thirdhand smoke, and it is much less studied than NNK and NNN in terms of its mutagenic potential," he said.

The researchers conclude in their paper: "Ultimately, knowledge of the mechanisms by which thirdhand smoke exposure increases the chance of disease development in exposed individuals should lead to new strategies for prevention."

Welcome to SUV System Ltd!

SUV System Ltd is ISO 90012008 Certified electronics distributor with 10 years of experiences.

We have built up long term business relationship with about many companies which are stockers and authorized agents. we have a steady and reliable supply to meet customer's demands to the greatest extent .Confidently, we are able to lower your cost and support your business with our years of professional service.

SUV System Ltd is Electronic Components Distributor Supplies,Find Quality Electronic Components Supplies Products IC(Integrated Circuits),Connectors,Capacitor,Resistors,Diodes,Transistors,LED at Suvsystem.com. Sourcing Other Energy, Environment, Excess Inventory Products from Manufacturers and Suppliers at Suvsystem.com

Electronic Components distributor:http://www.suvsystem.com

Connectors Distributor:http://www.suvsystem.com/l/Connectors-1.html

IC Distributor:http://www.suvsystem.com/l/IC(Integrated-Circuits)-1.html

LED Distributor:http://www.suvsystem.com/l/LED-1.html

Capacitor Distributor:http://www.suvsystem.com/l/Capacitor-1.html

Transistor Distributor:http://www.suvsystem.com/l/Transistors-1.html

Resistor Distributor:http://www.suvsystem.com/l/Resistors-1.html

Diode Distributor:http://www.suvsystem.com/l/Diodes-1.html

SUV System Ltd insists on the managing faith ofsincereness,speciality,foresight, win-win,so we build up stable-relationship customers located all over the world, including the States, Europe, Argentina, UAE, Malaysia, Australia,and India etc

we are focus on the following fields,and hope we can help you.

C0402C105Z9VAC C0402C105Z8VAC C0402C105M9PACTU C0402C105M9PAC C0402C105M8PACTU C0402C105M8PAC C0402C105M7PAC C0402C105K9PACTU C0402C105K9PAC C0402C105K8PACTU C0402C105K8PAC C0402C104Z9VACTU C0402C104Z9VAC7867 C0402C104Z9VAC C0402C104Z8VACTU C0402C104Z8VAC C0402C104Z4VACTU C0402C104Z4VAC C0402C104M9RACTU C0402C104M9RAC C0402C104M9PACTU C0402C104M9PAC C0402C104M8RACTU C0402C104M8RAC C0402C112K4GACTU C0402C112K3HACTU C0402C112K3HAC C0402C112K3GACTU C0402C112J5HACTU C0402C112J5HAC C0402C112J3HACTU C0402C112J3HAC C0402C111M5HAC C0402C111M3HAC C0402C111M1HAC C0402C111K5HACTU C0402C111K5HAC C0402C111K3HACTU C0402C111K3HAC C0402C111K1HACTU
http://www.suvsystem.com/a/3066.aspx